Generation of neutral pH high‐strength plasma‐activated water from a pin to water discharge and its bactericidal activity on multidrug‐resistant pathogens

Abstract

AbstractPlasma‐activated water (PAW) is emerging as a green alternative technology in biomedicine due to its rich and diverse aqueous reactive nitrogen and oxygen species (RONS). This study explores how to configure a pin to water (P2W) discharge to generate neutral pH high‐strength plasma‐activated water (hs‐PAW) and its bactericidal activity on hypervirulent multidrug resistance (MDR) pathogens. The factors affecting the strength of PAW, namely: water type, PAW temperature, plasma enclosure, and activation time, were studied and optimized to generate hs‐PAW having 650 ± 40 mg/l of and 215 ± 15 mg/l of H2O2 at a pH of 7. This study demonstrates a simple P2W discharge set up to handle the MDR pathogens and can be easily scalable for real‐world medical applications.