Abstract
We present a simple method using an axicon and spatial light modulator to create multiple parallel Bessel beams and precisely control their individual positions in three dimensions. This technique is tested as an alternative to classical holographic beam shaping commonly used now in optical tweezers. Various applications of precise control of multiple Bessel beams are demonstrated within a single microscope giving rise to new methods for three-dimensional positional control of trapped particles or active sorting of micro-objects as well as "focus-free" photoporation of living cells. Overall this concept is termed a 'biophotonics workstation' where users may readily trap, sort and porate material using Bessel light modes in a microscope.
Highlights
Propagation-invariant optical fields, in particular Bessel beams (BBs), have been studied intensively and are finding an increasing frequency of application in many areas of science [1]
The spatial spectrum modulation of an axicon-generated Bessel beam is a simple way to multiplex and control this beam for biophotonics applications
The supporting theory shows that spatial spectrum modulation can be applied regardless of which object field is used and for a combination of linear and quadratic phase modulations it always leads to 3-D positioning of the image field
Summary
Propagation-invariant optical fields, in particular Bessel beams (BBs), have been studied intensively and are finding an increasing frequency of application in many areas of science [1]. In this work we explore a different approach towards achieving this goal and in particular to exploit the spectral modulation of BBs, which, as we shall describe, is a more powerful generic concept than standard holographic beam shaping in this instance This concept was originally described by Bouchal[12, 13], where the phase modulation is applied on the annular spatial spectrum field (far-field) of a BB and can result in the splitting of the original BB into a number of parallel BBs where the position of each of them can be precisely and independently controlled. Overall this embodies the concept of Biophotonics Workstation where the user may use such Bessel modes to trap, sort and porate cellular material at will
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