Abstract
Abstract The development of effective approaches for not only the in vitro maturation (IVM) of heifer/cow oocytes and their extracorporeal fertilization (IVF) but also the non-surgical collection and transfer of bovine embryos has given rise to optimizing comprehensive in vitro embryo production (IVP) technology and improving other assisted reproductive technologies (ART s), such as cattle cloning by embryo bisection, embryonic cell nuclear transfer (ECNT) and somatic cell nuclear transfer (SCNT). The primary goal of the present paper is to demonstrate the progress and achievements in the strategies utilized for embryonic cell cloning and somatic cell cloning in cattle. Moreover, the current article is focused on recognizing and identifying the suitability and reliability of bovine cloning techniques for nutritional biotechnology, agri-food and biopharmaceutical industry, biomedical and transgenic research and for the genetic rescue of endangered or extinct breeds and species of domesticated or wild-living artiodactyl mammals (even-toed ungulates) originating from the family Bovidae.
Highlights
The development of the methods used for the in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes and of the techniques for non-surgical collection and transfer of bovine embryos has been instrumental in the elaboration of in vitro embryo production (IVP) technology in cattle and other assisted
This paper aimed to demonstrate the achievements of assisted reproduction in cattle using embryonic cell and somatic cell cloning
A study performed by Velásquez et al (2017) with elongated blastocysts obtained nine days after transfer of the “halves” and whole bovine blastocysts shows that bisection impacts the transcriptomes of these blastocysts, Production of cloned cattle for biotechnological, agricultural and transgenic research 743 mainly by downregulating key genes involved in the 1) remodelling and degradation of the extracellular matrix, 2) control of cell growth, 3) intracellular detoxification of products of oxidative stress, and 4) transport of metabolites between individual intracellular compartments and organelles
Summary
The development of the methods used for the in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes and of the techniques for non-surgical collection and transfer of bovine embryos has been instrumental in the elaboration of in vitro embryo production (IVP) technology in cattle and other assisted.
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