Abstract
When chopped porcine pulmonary arteries were incubated with calcium ionophore A23187 (1) in the presence of indomethacin there was a time dependent generation of a substance which produced contractions of superfused strips of guinea-pig ileum smooth muscle (GPISM) which were indistinguishable from those induced by LTD 4. This material however had a different retention time from LTD 4 when subjected to HPLC and co-chromatographed with synthetic LTE 4. In addition to LTE 4 a substance which had properties indistinguisable from those of LTB 4 when assayed on a combination of guinea-pig lung parenchymal strips (GPP) and GPISM (2) was generated from the pulmonary artery. This substance co-chromatographed with synthetic LTB 4. The adventitia and intima were the richest source of LTE 4, the adventitia releasing slightly more than the intima. The output of LTB 4 and LTE 4 was inhibited by 6,9-deepoxy-6,9-(phenylimino)- Δ 6,8 prostaglandin I 1 (U-60,257). Nordihydroguaiaretic acid (NDGA) inhibited the generation of LTE 4.
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