Abstract
Abstract N-Formylmethionyl-leucyl-phenylalanine (FMLP) and phorbol myristate acetate (PMA) stimulate a time- and concentration-dependent generation of hydrogen peroxide (H2O2) by human neutrophils. Maximum production of H2O2 occurred 15 and 30 min after cell contact with FMLP (0.1 – 100 μM) and PMA (0.32 – 10 ng/ml), respectively. Preincubation of neutrophils with cytochalasin B significantly increased FMLP- but not PMA-induced H2O2 generation. The amount of H2O2 generated by FMLP-stimulated neutrophils was significantly enhanced in the presence of extracellular calcium, whereas PMA-treated cells produced the same amount of H2O2 in the presence or absence of extracellular calcium. Trifluoperazine (TFP), an inhibitor of calmodulin, caused a doserelated, reversible, inhibition of FMLP- and PMA-elicited H2O2 generation. Carbobenzoxy-phenylalanyl—methionine (CBZ-Phe—Met), an antagonist of N-formylmethionyl peptide receptors, suppressed, in a dose-related manner, the production of H2O2 by neutrophils exposed to FMLP but not PMA.
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