Abstract
Using a selection system based on the two irreversible biochemical inhibitors, actinomycin-D and emetine hydrochloride, we have constructed human T-lymphocyte hybrids between the human T-cell line, Molt-4F, and mitogen-activated human lymphocytes. The cells were identified as true hybrids by their karyotype, differences in supernatant activities, responses to mitogen and membrane characteristics. These hybrid cells are stable in culture and express functions found in mature human T-lymphocytes.
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