Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA.

Abstract

Human-induced pluripotent stem cells (iPSCs) are showing great promise for both disease modeling and regenerative medicine. The choice of reprogramming methods have a significant effect on the outcomes of the experiments. Standard methods, such as Sendai viruses, episomes, and the base-modified mRNA have limitations. Here, I describe a method to reprogram human fibroblasts using a cocktail of mRNAs without any base modification that increases reprogramming efficiency, reduces the RNA-associated toxicity, and yields iPSCs ready for expansion and characterization in as short as 10-14days.