Abstract
Availability of numerous high-quality iPSC lines is needed to overcome donor-associated variability caused by genetic background effects. We generated two human iPSC lines from dermal fibroblasts of two healthy females using Sendai virus reprogramming. Quality assessment of the iPSC lines confirmed the expression of pluripotency markers, trilineage differentiation capacity and absence of exogenous expression of reprogramming factors. Both iPSC lines were genetically stable with a genotype that matched the fibroblast lines of donors. These iPSC lines add to available reference lines as a resource for disease modeling of polygenic and multifactorial diseases, for evaluation of differentiation protocols and toxicology screening.
Highlights
Availability of numerous high-quality induced pluripotent stem cells (iPSC) lines is needed to overcome donor-associated variability caused by genetic background effects
Resource utility The application of patient-derived induced pluripotent stem cells in biomedical research is dependent on high-quality reference iPSC lines
The iPSC lines from two healthy females increase the number of well-characterized control lines as a resource for disease modeling and genome editing, evaluation of cell differentiation protocols and toxicology screening
Summary
The application of patient-derived induced pluripotent stem cells (iPSC) in biomedical research is dependent on high-quality reference iPSC lines. The iPSC lines from two healthy females increase the number of well-characterized control lines as a resource for disease modeling and genome editing, evaluation of cell differentiation protocols and toxicology screening. Studies based on several iPSC lines of different biological origins reduce the risk for genetic background effects
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