Abstract

A method for the generation of concentration gradients using two exponential dilution flasks in series is described. The use of two flasks rather than one allows the generation of a gradient which starts at zero, rises to a maximum and then returns to zero, rather than one which starts at a maximum and then goes to zero. In addition to the normal use of a single exponential dilution flask for calibration, this method also allows the study of systems which may exhibit hysteresis effects.

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