Abstract
The availability of otherwise not readily accessible intraocular cells via induced pluripotent stem cell (iPSC) technology offers great potential for disease modelling, drug screening, and cell-based transplantation therapy in degenerative ocular disorders. Direct differentiation of iPSCs into retinal pigment epithelium (RPE) is particularly straightforward, and iPSC-derived RPE cell cultures have been demonstrated to yield pure populations of functional cells that display many features of native RPE. Here, I describe a protocol for the generation of iPSC-derived RPE monolayer, their propagation, and cryostorage. A reliable monitoring for functional cell differentiation is achieved by measuring transepithelial resistance.
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