Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

Chih-Hsien Wu,Hsuan-Hwai Lin,Yi-Lin Chiu,Tsung-Neng Tsai,Chung-Chi Yang,Li-Yen Huang,Yi-Ying Wu,Cheng-Chung Cheng

doi:10.3390/biomedicines9111565

Abstract

Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.

Highlights

Coronary artery disease (CAD) is a major cause of death worldwide
The present study is the first report of IPSCs derived from Human gastric fibroblast cells (hGFCs) that were obtained from PES biopsy
We established a protocol to obtain hGFCs and generate induced pluripotent stem cell (iPSC) via tiny gastric tissue collected from PES with biopsy

Summary

Introduction

Coronary artery disease (CAD) is a major cause of death worldwide. Aspirin has been used as a first-line treatment for CAD patients according to the American Heart Association and European Society of Cardiology guidelines. The progression of CAD can lead to ischemic cardiomyopathy and eventually heart failure Half of these patients will die within 5 years due to poor life quality. Gastric fibroblasts can secrete proteins, which are the main sources of extracellular matrix of the stomach. They play a critical role in regulating epithelial cell activities, such as proliferation and restitution [1]. Somatic cells have been reprogrammed by introducing four genes to revert them into induced pluripotent stem cells (iPSCs), which provide an alternative to using embryonic stem cells (ESCs) [2,3] These cells share numerous characteristics with ESCs, such as self-renewal to persist in their undifferentiated state and differentiation potential to specialized cell types, but without the technical

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