Generation of Fluorescent and Stable Conjugated Polymer Nanoparticles with Hydrophobically Modified Poly(acrylate)s

Abstract

Conjugated polymer nanoparticles hold promise as fluorescence imaging agents because of their high brightness and photostability. However, their aggregation and precipitation at the high ionic strengths in physiological conditions present an impediment to their applications in biological imaging. In this study, hydrophobic linear alkyl group substituted poly(acrylate)s, PAACn, are synthesized and employed as amphiphilic stabilizers. The carboxylate groups of PAACn increase the surface charge of conjugated polymer nanoparticles, stabilizing the particles in phosphate buffer saline, PBS. Nanoparticles of the green-yellow emitting conjugated polymer, F8BT, stabilized with PAACn (F8BT-PAACn) are prepared using nanoprecipitation. In contrast to the significant aggregation with a negligible yield (∼0%) of bare F8BT nanoparticles in PBS, high yields approaching 90% are observed for F8BT nanoparticles stabilized with PAAC18 at 1%, PAAC16 at 3%, and PAAC10 at 10% substitution. The F8BT-PAACn nanoparticles have a s...