Abstract

The present study aimed at isolation characterization and evaluation of electrogenic bacteria for electricity generation using waste water. In this context, waste water samples were collected from University of Nizwa waste water treatment plant. A total of eight distinct bacterial isolates were isolated from these samples by serial dilution and plating on LB Agar medium. The bacterial isolates were than grown at different temperatures and pH. DNA from bacterial samples was isolated and 16S rRNA gene amplification was carried out. The 16S rRNA gene PCR products were directly sequenced and the resulting sequence was blasted using BLASTn. Based on BLAST results, the bacterial strains were identified. The bacteria were used in different combinations to generate electricity from waste water in microbial fuel cells constructed using plastic bottles. The microbial isolates were found to produce varying levels of currents and their electrogenic potential in waste water was observed to increase with the passage of time.

Highlights

  • The world energy demand and the corresponding need to find energy sources alternative to fossil fuels are on an ongoing increase

  • How to cite this paper: Naureen, Z., Al Matani, Z.A.R., Al Jabri, M.N., Al Housni, S.K., Gilani, S.A., Mabood, F., Farooq, S., Hussain, J. and Al Harrasi, A. (2016) Generation of Electricity by Electrogenic Bacteria in a Microbial Fuel Cell Powered by Waste Water

  • The present study will focus on electrogenic potential of facultative anaerobic bacteria to generate the electricity in a fabricated two-chambered MFC powered by waste water

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Summary

Introduction

The world energy demand and the corresponding need to find energy sources alternative to fossil fuels are on an ongoing increase. Wastewater is an energy rich source for growth of several anaerobic and facultative bacterial species which have the capability to transfer electrons to an anode, as a terminal electron acceptor and are classified as electrogenic bacteria (EB; [3]-[6]). These EB have been documented to generate electricity by assimilating a variety of sources [7]-[10] like carbohydrates [11], textile effluents [12], waste matter in land fill [1], sea sediments and waste water [1]; a very few reports are available where waste water is used as fuel for electrogenic bacteria which utilize organic and inorganic compounds in it as fuel to generate electricity. The present study will focus on electrogenic potential of facultative anaerobic bacteria to generate the electricity in a fabricated two-chambered MFC powered by waste water

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