Abstract
Background:Dengue virus (DENV) infection is common around the world, especially in tropical and subtropical areas. DENV infection can cause from milder dengue fever (DF) to severe dengue hemorrhage fever (DHF) and dengue shock syndrome (DSS). Because the lack of effective drugs or vaccines against DENV, early detecting of DENV infection become an important issue and can provide supportive treatment for dengue patients as soon as possible which can reduce the threats of DENV infection. Recent studies have proved that DENV nonstructural protein 1 (NS1) circulates in blood and can be serve as early marker of the infection. Methods:RecombinantNS1 fromDENV type 2 strain PL046was used to immunize BALB/c mice to generate monoclonal antibody (mAb) specifically against NS1. Enzyme-linked immunosorbent assay (ELISA), western blot, immunofluorescence assay (IFA) and flow cytometry analysis were used to detect NS1 in DENV infected cells. Results: We got 5 mAbs which could recognize recombinant NS1 by ELISA and DENV-infected cells by flow cytometry. Further, analysis showed that these mAbs could recognize 1-3 different DENV serotypes by IFA. Application of thesemAbs could also detect native NS1 in DENV infected cells by different methods such as ELISA, flow cytometry and western blot. Conclusion: Taken together, we have generated mAbs against NS1 which could be used to detect NS1 in DENV infected samples by different methods.
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