Abstract
Fluorescent in situ hybridization (FISH) has become a widespread technique applicable in basic science and diagnostics. Chromosome painting represents a special application of FISH that has found increasing use in identification of complex chromosome rearrangements. Here we present a version of the Alu-PCR method modified to generate a whole chromosome painting probe (WCP) for human chromosome 19 using monochromosomal cell hybrids. In setting up conditions for this method, we established a cheap and fast approach to generation of WCPs for other human chromosomes that could be particularly useful for unambiguous identification of complex chromosomal rearrangements associated with cancer. .
Highlights
Fluorescent in situ hybridization is a powerful method that permits visual identification of specific nucleic acid sequences in metaphase or interphase cells (Trask, 1991; Pinkel et al, 1986)
In addition to routine cytogenetic analysis, where karyotyping is the most conclusive test for chromosome analysis, Fluorescent in situ hybridization (FISH) technique has been introduced in order to achieve a higher sensitivity, specificity, and resolution of chromosomes (Trask, 1991)
In order to apply FISH for rapid detection of chromosomal translocations, we used the method of chromosome painting
Summary
Fluorescent in situ hybridization is a powerful method that permits visual identification of specific nucleic acid sequences in metaphase or interphase cells (Trask, 1991; Pinkel et al, 1986). The given approach served as a basis for extraction of human DNA from interspecific somatic cell hybrids, which permitted production of chromosome- and chromosome region-specific probes for FISH (L i u et al, 1993). We present a version of the Alu-PCR method modified for production of WCP 19 using monochromosomal cell hybrids.
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