Abstract
Reporter influenza A viruses (IAVs) carrying fluorescent or luminescent genes provide a powerful tool for both basic and translational research. Most reporter IAVs are based on the backbone of either subtype H1N1 viruses, A/Puerto Rico/8/1934 (PR8) or A/WSN/1933, but no reporter subtype H3N2 virus is currently available to our knowledge. Since the IAV subtype H3N2 co-circulates with H1N1 among humans causing annual epidemics, a reporter influenza A subtype H3N2 virus would be highly valuable. In this study, the segments of A/Wyoming/3/03 (NY, H3N2) virus encoding hemagglutinin and neuraminidase, respectively, were reassorted with the six internal genes of PR8 where the NS gene was fused with a Gaussia luciferase (Gluc) gene. Using reverse genetics, NY-r19-Gluc, a replication competent reassortant influenza A subtype H3N2 virus expressing reporter Gluc was successfully generated. This reporter virus is stable during replication in Madin-Darby canine kidney (MDCK) cells, and preliminary studies demonstrated it as a useful tool to evaluate antivirals. In addition, NY-r19-Gluc virus will be a powerful tool in other studies including the application of diagnostic and therapeutic antibodies as well as the evaluation of novel vaccines.
Highlights
The influenza A virus (IAV) is a major cause of respiratory illness in humans, accounting for up to650,000 annual deaths worldwide [1]
The IAV belongs to the family Orthomyxoviridae of enveloped viruses, and the genome consists of 8 negative-sense, single-stranded viral RNA segments, coding for at least 11 proteins [2]
Reporter Influenza A Subtype H3N2 Virus with a Gaussia luciferase (Gluc) Gene Fused to NS1 Loses the Reporter
Summary
The influenza A virus (IAV) is a major cause of respiratory illness in humans, accounting for up to. 650,000 annual deaths worldwide [1]. The IAV belongs to the family Orthomyxoviridae of enveloped viruses, and the genome consists of 8 negative-sense, single-stranded viral RNA (vRNA) segments, coding for at least 11 proteins [2]. Hemagglutinin (HA) and neuraminidase (NA) are the major surface glycoproteins, based on which IAVs can be classified into different subtypes. There are 16 subtypes of HA (H1–H16) and 9 subtypes of NA (N1–N9) identified in wild birds [3]. Two new subtypes (H17N10 and H18N11) have recently been isolated in bats [4,5].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
