Abstract
The relationship between T-antigen interactions at Site I and the presence of a nucleosome-free promoter in SV40 chromatin was examined by analyzing chromatin from mutants defective for T-antigen interaction at site I (cs1085, scs1 11, and tsA58) and their parental wild-type strains (776, SVS, and VA45–54, respectively). As judged by sensitivity to digestion with restriction endonucleases that recognize unique sequences within the promoter region ( Bg/I, KpnI, and MspI), a nucleosome-free promoter was observed in a substantially larger proportion of chromosomes from the defective mutants than their wild-type parents. This result demonstrates that T-antigen binding to site I is not necessary for setting the early boundary of the nucleosome-free region, although it may function directly or indirectly in determining the proportion of chromosomes containing this feature.
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