Abstract
The properties of the focal spot for 4pi focusing with radially polarized first-order Laguerre-Gaussian beams are calculated. It is shown that a focal spot that has an extremely sharp dark region at the center and an almost-perfect spherical symmetry can be achieved. When such a hollow dark spherical spot is used in 4pi fluorescence depletion microscopy, an axial FWHM spot size of approximately 39 nm and a transverse FWHM spot size of approximately 64 nm can be achieved simultaneously in a practical system.
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