Generation mean analysis of spring wheat (Triticum aestivum L.) seedlings tolerant to high levels of manganese

Abstract

Effective utilisation of wheat (Triticum aestivum L.) germplasm tolerant to high manganese (Mn) in a breeding program requires an understanding of the genetics of Mn tolerance. Five wheat cultivars differing in their response to high Mn were crossed in a half diallel design with no reciprocals. Seedlings of parents and progeny generations were phenotyped in high Mn nutrient solutions. Total chlorophyll concentration, which was positively correlated with leaf elongation rate during recovery from Mn stress, was used for phenotyping Mn tolerance. Means of chlorophyll concentration of P1, P2, F1, F2, BC1, and BC2 generations, in all crosses which showed significant variation among generations, were subjected to line crosses analysis to estimate gene effects. The continuous frequency distribution of seedlings with differential Mn tolerance, the similarity of the F1 and F2 means, and the high and significant levels of additive gene action indicated quantitative inheritance for Mn tolerance at seedling stage. A preponderance of additive effects indicated that selection for Mn tolerance in early generations should be effective.