Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a patient with alcoholic liver cirrhosis of Indian ethnicity.

Mohsina Khan,Anjali Shiras,Deepika Suresh,Arun Vaidyanath,Bhumika Vaishnav,Arjun Lal Kakrani,Swapnil Patil

doi:10.1016/j.scr.2019.101678

Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a patient with alcoholic liver cirrhosis of Indian ethnicity.

Mohsina Khan, Anjali Shiras + Show 5 more

Open Access

https://doi.org/10.1016/j.scr.2019.101678

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Journal: Stem Cell Research	Publication Date: Dec 16, 2019
Citations: 2	License type: cc-by

Affiliation: National Centre for Cell Science, Dr. D.Y. Patil Vidyapeeth, Pune

#iPSC Clones #Liver Cirrhosis + Show 8 more

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Abstract

Three induced pluripotent stem cells (iPSC) clones NCCSi007-A, NCCSi007-B and NCCSi007-C were generated from CD4+T cells of a 38 years old male patient suffering from liver cirrhosis- alcoholic and minimal hepatic encephalopathy of Indian origin. The CD4+T cells of the patient were reprogrammed using integration free, Sendai viral vector system. Each of the three iPSC clones showed high alkaline phosphatase (ALP) activity, expressed pluripotency markers OCT4, SOX2, NANOG, KLF4, SSEA-4, TRA-1-60, showed normal male karyotype (46, XY) and exhibited multi-lineage differentiation.

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