Abstract
Many nuclear proteins are transported into the nucleus via the importin α/β-mediated pathway. Importin α comprises a multigene family. In this study, we generated and characterized a rat monoclonal antibody (MAb) 3F8 to importin α7. The antibody was generated by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat. The MAb 3F8 specifically recognized importin α7 among importin α isoforms as evidenced by immunoblotting analysis. Furthermore, MAb 3F8 detected exogenous importin α7 in COS-7 cells by immunofluorescence. This MAb will be useful in the analysis of the isoform-specific function of importin α7.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
