Abstract
Meflin (Islr) expression has gained attention as a marker for mesenchymal stem cells, but its function remains largely unexplored. Here, we report the generation of Meflin-CreERT2 mice with CreERT2 inserted under the Meflin gene promoter to label Meflin-expressing cells genetically, thereby enabling their lineages to be traced. We found that in adult mice, Meflin-expressing lineage cells were present in adipose tissue stroma and had differentiated into mature adipocytes. These cells constituted Crown-like structures in the adipose tissue of mice after high-fat diet loading. Cold stimulation led to the differentiation of Meflin-expressing lineage cells into beige adipocytes. Thus, the Meflin-CreERT2 mouse line is a useful new tool for visualizing and tracking the lineage of Meflin-expressing cells.
Highlights
White adipose tissue (WAT) is responsible for the storage and release of energy in response to environmental changes
We confirmed the occurrence of recombination with CreERT2 throughout the body and observed that many lineage cells were located in the stroma
De novo adipogenesis in gonadal WAT (gWAT) reportedly occurs in fibroblasts in an outer structure, known as the capsule, that surrounds adipose tissue [22]
Summary
White adipose tissue (WAT) is responsible for the storage and release of energy in response to environmental changes. Increases in the number of adipocytes can occur through the clonal proliferation of mature adipocytes and the differentiation of preadipocytes, which are the precursor cells of adipocytes [3]. The mechanism of differentiation from mesenchymal stem cells, which are thought to be more immature than preadipocytes, to the adipocyte lineage is still unclear. Meflin (Islr) expression has received much attention as a marker for mesenchymal stem cells. The gene expression patterns of preadipocytes and more immature cells in adipose tissue have been analyzed and clarified at the single cell level. We analyzed how mesenchymal stem cells and their progeny differentiate into cells in adipose tissue in adults. Meflin-expressing lineage cells were found in multivesicular, brownish-toned cells, suggesting their differentiation into cells that were thought to be beige adipocytes
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