Abstract
This paper introduces a widely accessible and straightforward technique for fabricating membrane protein arrays. This technique employs topographically patterned agarose gels to deliver various membrane preparations to glass substrates in a rapid and parallel fashion. It can fabricate more than 30 identical copies of a membrane protein array while requiring only femtomoles of protein. Taking advantage of on-stamp preconcentration, it is able to pattern arrays of multilayered membrane spots with more than 20-fold increased content of membrane proteins compared to existing methods.
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