Abstract
The immunological properties of fat-soluble (Cernitin GBX) and water-soluble (Cernitin T60) pollen extracts were examined in vivo and in vitro. For investigations in vitro we used the water-soluble pollen extract (T60), and in vivo the fat-soluble form (GBX). The aim of the in vivo experiments was to evaluate their effect on IgG antibody production, their capability of rosette formation (E-RFC) and cell indicating IgM-plaqueforming cells (PFC) hemolysins. Also observations were made on the effect of the pollen extract on graft vs host reaction, the transplantation barrier and delayed hypersensitivity in relation to sheep red blood cells (SRBC). Its assumed effect on phagocytosis and blastic transformation was assessed in vitro. In both in vivo and in vitro systems the influence of T60 and GBX preparations on the population of T- and B-cells was tested. A relationship was revealed between immunological activity and the evaluated Cernitins. Both the Cernitins examined demonstrated moderate immunoregulatory properties, but the immunosuppressive component was predominant.
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