Abstract
A new flow-through sensor for the determination of hydrogen peroxide based on the dual immobilisation of the catalyst and the reaction product (permanent immobilisation in a reactor and transient retention in the flow-cell, respectively) is proposed. The support packed in the flow-cell is an aminopropyl-bonded silica gel non-fluorescent material. The approach provides a linear range between 0.03–17 ng ml–1 H2O2 (r2 = 0.9954, RSD values less than 3.5%). This sensor is 80 times more sensitive than a photometric flow-through biosensor previously reported and based on similar principles.
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