Abstract
An alkalophilic strain of Bacillus sp., designated TS-23, was isolated from a soil sample collected at a hot spring (Tainan, Taiwan). During growth in a medium containing 1% soluble starch as the sole source of carbon, the fermentation broth exhibited both pullulanase and amylase activity. Pullulanase and amylase activities were maximal at 65° C. The pH optima were 8.8 to 9.6 for pullulanase and 7.5 to 9.4 for amylase. Under optimal conditions, a crude preparation hydrolysed pullulan, generating maltotriose as the major product. Strain TS-23 was found to produce five amylases (Ac, A1, A2, AP1, and AP2), which were visualized by activity staining of proteins that had been separated by native polyacrylamide gel electrophoresis. Both AP1 and AP2 had pullulanase activity and Ac, A1 and A2 had the ability to adsorb to raw corn-starch. Native corn-starch was partially digested by adsorbed amylases during the course of 12 h at 50° C, with initiation of granular pitting. Further incubation of the reaction mixture resulted in considerable morphological changes in corn-starch granules, and the main soluble products were maltose, maltotriose and higher oligosaccharides.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
