Abstract
The tomato crop is exposed to serious losses due to infection with several diseases and pests, which threaten tomato production in Egypt and worldwide. Therefore, selecting the tomato germplasm resistant to a specific pathogen by molecular markers closely linked to resistance loci is a desirable goal of this study. In this work, seven co-dominant markers targeting six resistance genes (I-1, Ve, Ph3, Cf-9/Cf-4, Rx4, and Pto) for six main diseases [ fusarium wilt (Fusarium oxysporum f. sp. lycopersici), verticillium wilt (Verticillium dahliae and V. alboatrum), late blight (Phytophthora infestans), leaf mold (Cladosporium fulvum), bacterial spot (Xanthomonas campestris pv. vesicatoria) and bacterial speck (Pseudomonas syringae pv. tomato)], respectively were determined. Theses molecular markers differentiated among 19 tomato genotypes resistant (homozygote/heterozygote) and susceptible (homozygote) to the pathogens. Therefore, this study supplied us with novel tomato lines with resistance to multiple diseases, and their pyramiding inside domesticated tomato cultivars are suggested to apply in the tomato breeding programs of resistance against fungal and bacterial diseases.
Highlights
Breeding for biotic stress resistance in the plants is considered one of the most crucial ways in the breeding programs
Tomato (Solanum lycopersicum L.), one of the most important horticultural crops of Egypt and worldwide. It is infected with many fungal and bacterial diseases e.g., wilt disease caused by Fusarium oxysporum f. sp. lycopersici, verticillium wilt (Verticillium dahliae and V. alboatrum), late blight (Phytophthora infestans), leaf mold (Cladosporium fulvum), bacterial spot (Xanthomonas campestris pv. vesicatoria) and bacterial speck (Pseudomonas syringae pv. tomato) are a dangerous threat to tomato farming (Lee et al, 2015)
The purpose of this study was to identify the resistance alleles corresponding to fusarium wilt, verticillium wilt, late blight, leaf mold, bacterial spot, and bacterial speck of 19 tomato genotypes by molecular markers, which will be used as marker-assisted selection (MAS) in tomato breeding programs
Summary
Breeding for biotic stress resistance in the plants is considered one of the most crucial ways in the breeding programs. The use of molecular markers in the identification and characterization of resistance genes has become an important tool, because they are not affected by environmental conditions. Molecular markers supply a unique chance to select a big number of germplasms in a short time. A big number of gene-based markers have been identified in various crops, including tomato (Foolad, 2007). Tomato (Solanum lycopersicum L.), one of the most important horticultural crops of Egypt and worldwide. It is infected with many fungal and bacterial diseases e.g., wilt disease caused by Fusarium oxysporum f. There are a big number of tomato germplasms, many resistance loci for various diseases have been reported (van Ooijen et al, 2007). Molecular markers become an important tool in the tomato breeding
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