Abstract
We evaluated the transfection efficiency of five different cationic liposome/plasmid DNA complexes, during the in vitro gene transfer into human epithelial tracheal cell lines. A dramatic correlation between the transfection efficiency and the charge ratio (positive charge of liposome to negative charge of DNA) has been found. DC-Chol–DOPE was found to be the most effective liposome formulation. Therefore, a morphological and structural analysis of DC-Chol–DOPE liposomes and DC-Chol–DOPE/DNA complexes, has been performed by transmission electron microscopy (TEM) and by confocal laser scanning microscopy (CLSM), respectively. The process of interaction between DC-Chol–DOPE/DNA complexes and human epithelial tracheal cells has been studied by CLSM. These results raise some issues for in vivo gene therapy.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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