Gene transcription; a practical approach: Edited by B. D. Hames and S. J. Higgins. 364 pp. 1993. IRL/Oxford University Press, Oxford. HB $60, PB $42

Abstract

Part 1 Assay of gene transcription vitro, Philip J. Mason et al: northern transfer RNA slot blots analysis of specific RNA molecules by polymerase chain reaction (PCR) S1 nuclease mapping RNase mapping primer extension situ hybridization. Part 2 Transcription of exogenous genes in mammalian cells, Kevin Docherty and Andrew R. Clark: introduction of exogenous DNA into mammalian cells in culture transient and stable expression vectors for introducing DNA into mammalian cells in culture the characterization of transcriptional regulatory sequences mutagenesis of reporter gene constructs. Part 3 In vitro transcription with nuclear extracts differentiated tissues, Felipe Sierra et al: general practical considerations preparation of transcriptionally-active nuclear extracts from solid rat tissues analysis of vitro transcripts factors affecting the efficiency of vitro transcription trouble-shooting. Part 4 Transcriptional analysis using transgenic animals, Niall Dillon and Frank Grosveld: introduction - transgenic mice generation of transgenic mice setting up a transgenic operation for the analysis of gene function isolation of DNA for micro-injection integration of injected genes generation of transgenic mice using embryonic stem cells breeding of transgenic animals design of transgenic experiments other transgenic systems. Part 5 Identification and characterization of eukaryotic transcription factors, Stephen P. Jackson: DNA binding assays for identifying transcription factors in crude cell extracts identifying the DNA-binding polypeptide purification of sequence-specific transcription factors using DNA binding site affinity chromatography determining the purity and activity of a purified transcription factor problems and trouble-shooting isolation of cDNA clones encoding transcription factors post-translational modification of transcription factors. Part 6 Analysis of protein-DNA interactions, Michael J. Garabedian et al: the mobility shift DNA-binding assay for protein-DNA interactions assays for sites of protein-DNA contact - footprinting techniques methylation interference assay random mutagenesis of DNA sequences purification of DNA-binding proteins. Part 7 Cloning and fuctional analysis of heterologous eukaryotic transcription factors in yeast, Daniel M. Becker and John D. Fikes: expression cloning of heterologous transcription factors in yeast analysis of heterologous transcription factors in yeast. Appendices: transcription controls - cis-elements and trans-factors, Joseph Locker fractionation of nucleic acids by gel electrophoresis, B.D. Hames and S.J. Higgins addresses of suppliers.