Abstract
A goose-type lysozyme ( g-lysozyme) gene has been cloned from the mandarin fish ( Siniperca chuatsi), with its recombinant protein expressed in Escherichia coli. From the first transcription initiation site, the mandarin fish g-lysozyme gene extends 1307 nucleotides to the end of the 3′ untranslated region, and it contains 5 exons and 4 introns. The open reading frame of the g-lysozyme transcript has 582 nucleotides which encode a 194 amino acid peptide. The 5′ flanking region of mandarin fish g-lysozyme gene shows several common transcriptional factor binding sites when compared with that from Japanese flounder ( Paralichthys olivaceus). The recombinant mandarin fish g-lysozyme was expressed in E. coli by using pET-32a vector, and the purified recombinant g-lysozyme shows lytic activity against Micrococcus lysodeikticus.
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