Gene Structure of Drosophila Diaphorase-1 the Diversity of the Transcripts in Female and Male Adult Flies

Abstract

ABSTRACTThe gene DMC22E5 (ac. # CG4199) from Barkely Drosophila Genome Project (BDGP) ((www.fruitfly.org) was found, using the partial amino acid sequences of three tryptic peptides, obtained from purified Drosophila virilis Diaphorase—1,. This gene is located on the X chromosome at position 2C9-2C10. The structure of the gene reveals three exons and two long introns. Using Barkely Drosophila Genome Project (BDGP) we found six transcripts from this gene (ac.# CG4199RF, CG4199RC, CG4199RD, CG4199RB, CG4199RE, CG4199RA). The difference between these transcripts is in their 5' untranslated regions (UTR) and their 3' ends are identical. Thirty-four ESTs (expressed sequence tags) from different cDNA libraries were found in the collection of the ESTs. Most of them are from Schnaider L2 cell culture (SH) cDNA library. The transcripts are represented at vary law level in the cells of different organs and at different stages of Drosophila development. Using RT-PCR we obtained the five of these transcripts in cDNA samples from female adult flies. We couldn't find any of them in cDNA samples from male adult flies.