Gene structure of carp Cyprinus carpio cathepsin B

Abstract

The 2474 nucleotides of carp cathepsin B gene (corresponding to the part of open reading frame and 3′non-coding region of cDNA) have been determined by polymerase chain reaction cloning, which was organized into nine exons and eight introns. The boundary sequences of the exon-intron junctions conformed to the GT/AG consensus rule. One polyadenylation signal sequence of AATAAA was found in the 3′non-coding region. The sizes of the determined introns were approximately 100–200 bp in length (varied from 71 bp-239 bp), which were significantly shorter than those of mouse cathepsin B gene.