Abstract
BackgroundMagnaporthe oryzae, the causal agent of blast disease of rice, is the most destructive disease of rice worldwide. The genome of this fungal pathogen has been sequenced and an automated annotation has recently been updated to Version 6 . However, a comprehensive manual curation remains to be performed. Gene Ontology (GO) annotation is a valuable means of assigning functional information using standardized vocabulary. We report an overview of the GO annotation for Version 5 of M. oryzae genome assembly.MethodsA similarity-based (i.e., computational) GO annotation with manual review was conducted, which was then integrated with a literature-based GO annotation with computational assistance. For similarity-based GO annotation a stringent reciprocal best hits method was used to identify similarity between predicted proteins of M. oryzae and GO proteins from multiple organisms with published associations to GO terms. Significant alignment pairs were manually reviewed. Functional assignments were further cross-validated with manually reviewed data, conserved domains, or data determined by wet lab experiments. Additionally, biological appropriateness of the functional assignments was manually checked.ResultsIn total, 6,286 proteins received GO term assignment via the homology-based annotation, including 2,870 hypothetical proteins. Literature-based experimental evidence, such as microarray, MPSS, T-DNA insertion mutation, or gene knockout mutation, resulted in 2,810 proteins being annotated with GO terms. Of these, 1,673 proteins were annotated with new terms developed for Plant-Associated Microbe Gene Ontology (PAMGO). In addition, 67 experiment-determined secreted proteins were annotated with PAMGO terms. Integration of the two data sets resulted in 7,412 proteins (57%) being annotated with 1,957 distinct and specific GO terms. Unannotated proteins were assigned to the 3 root terms. The Version 5 GO annotation is publically queryable via the GO site . Additionally, the genome of M. oryzae is constantly being refined and updated as new information is incorporated. For the latest GO annotation of Version 6 genome, please visit our website . The preliminary GO annotation of Version 6 genome is placed at a local MySql database that is publically queryable via a user-friendly interface Adhoc Query System.ConclusionOur analysis provides comprehensive and robust GO annotations of the M. oryzae genome assemblies that will be solid foundations for further functional interrogation of M. oryzae.
Highlights
Magnaporthe oryzae, the rice blast fungus, infects rice and other agriculturally important cereals, such as wheat, rye and barley
In order to maximize the utility of the genome sequence, we have developed manual curations of the predicted genes
36 proteins were annotated with Gene Ontology (GO):0005975 ("carbohydrate metabolic process"), and 12 proteins were annotated with GO:0006520 ("amino acid metabolic process")
Summary
Magnaporthe oryzae, the rice blast fungus, infects rice and other agriculturally important cereals, such as wheat, rye and barley. The rice blast fungus has been the focus of intense investigation and a number of genomic resources have been generated. Numerous genes have been functionally characterized by targeted knockout [7,8,9,10,11,12,13,14,15,16,17,18] While these resources are of tremendous utility, much of the genome remains unexplored. Magnaporthe oryzae, the causal agent of blast disease of rice, is the most destructive disease of rice worldwide The genome of this fungal pathogen has been sequenced and an automated annotation has recently been updated to Version 6 http://www.broad.mit.edu/annotation/genome/ magnaporthe_grisea/MultiDownloads.html. We report an overview of the GO annotation for Version 5 of M. oryzae genome assembly
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