Gene network analysis of Diabetic Nephropathy and its associated diseases- An in silico study

Abstract

Metabolic syndrome (MetS) is a cluster of conditions including Diabetic nephropathy (DN), Hypertension (HT), Type 2 Diabetes mellitus in obese and obesity. Network biology has been developed as a way to review gene interactions. Herein an effort was made to identify common genes between the above MetS using Cytoscape3.0. Further molecular docking study has been performed to identify potential inhibitors for common genes using active phytoconstituents of Pueraria tuberosa (PTY-2). AGT (angiotensinogen) and ACE (angiotensinconverting enzyme) were found to be common genes between DN and HT. From the molecular docking study, tuberosin (9.19 kcal/mol) and robinin (11.28 kcal/mol) showed highest binding affinity with AGT and ACE and followed all drug-like properties as per Lipinski’s rule of five and Admet prediction. Hence, based on our findings we may suggest that by inhibiting AGT and ACE, the downstream functioning pathways are responsible for these diseases and their associated complications can be normalized.