Abstract
ABSTRACTOral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide, which appears as a consequence of multiple molecular genetic events in various chromosomes and genes. In order to unveil the possible mechanisms underlying OSCC tumorigenesis, the OSCC-related gene expression variance and the gene interaction network should be further investigated. Herein, we conducted the NimbleGen Human Gene Expression Microarray to analyze expression heterogeneity between OSCC primary tumor tissue and its adjacent normal tissue from two patients. A total number of 7872 out of 32,448 detected genes are differentially expressed in OSCC. Gene ontology (GO) analysis demonstrated that these differentially expressed transcripts were critical in a series of metabolic processes, cancer-related signal pathways, and biological regulations. KEGG signaling pathway enrichment suggested a number of pathways (metabolic process and immune response) which are frequently enrolled during cancer progression. 15 most differential regulated genes between OSCC tumor and non-tumor were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, the interaction network analysis of these confirmed genes by STRING database showed the two subunits of RACK1 had direct interaction with 14 differential proteins. This bioinformatics research lends support about the critical role of RACK1 which functions as a key node protein driving OSCC development.
Highlights
Oral squamous cell carcinoma (OSCC) is among the most lethal malignancies with 30,000 new cases diagnosed and approximately 11,000 deaths every year (Chaturvedi et al 2008; Wang et al 2013)
We have performed the microarray of two pairs of clinical samples of OSCC tumor and adjacent normal tissue to explore the aberrant expression of OSCC related genes and try to depict the gene-gene interaction network
We have figured out a number of significantly deregulated genes between OSCC tumor and non-tumor which is potential to link tumor initiation and malignancy
Summary
Oral squamous cell carcinoma (OSCC) is among the most lethal malignancies with 30,000 new cases diagnosed and approximately 11,000 deaths every year (Chaturvedi et al 2008; Wang et al 2013). Early-to-moderate-stage OSCC (American Joint Committee on Cancer stages I-III) is often treated surgically, with radiotherapy given in the presence or absence of chemotherapy in the postoperative adjuvant setting for high-risk patients(Lo et al 1976). In advanced (stage IV) cases, multidisciplinary non-surgical approaches are being applied with increasing frequency to improve disease control, prolong survival, and maintain life quality for patients. Even when the appropriate combination of surgical and non-surgical approaches is used, more than half of patients still experience cancer recurrence (Massano et al 2006). Recurrent and distantly metastatic OSCC carry poor prognosis (Johnson et al 1992). It is necessary to conduct studies on OSCC mechanisms to develop more effective and efficient therapies
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