Abstract
Phospholipasase Cs (PLCs) has been recognized as important enzymes for their roles in regulation, signal transduction and membrane composition. Two full length wheat PLCs cDNAs, Ta-PG-PLC2-1 and Ta-PG-PLC2-2 from wheat, Triticum aestivum L., were sequenced and mapped to wheat chromosome arms using PCR amplification in diploid wheat ancestors, Triticum urartu, Aegilops speltoides, Aegilops tauschii and cytogenetic stocks of Triticum aestivum and found to have very high sequence similarity and one, PG-PLC2-1, was found to contain a frame shift mutation 29 nt downstream of the first ATG start codon. This sequence was used to survey the prevalence of the mutation in twenty one wheat cultivars and was found to be present in all wheat cultivars tested and in the ancestral diploid species, Triticum urartu.
Highlights
The main objectives of this study were to determine the chromosomal location of the Phospholipasase Cs (PLCs) genes in wheat and survey the prevalence of a frame shift mutation identified in one Ta-PG-PLC gene, while sequencing, among twenty-one wheat cultivars and in progenitor species
Ta-PG-PLC2-1 clone is 1734 bp in length (Fig. 1) with a 1568 bp open reading frame extending from position 61 to position 1629
Ta-PGPLC2-1 has a frame shift mutation, a deletion of two bases following the G at position +30 relative to the first ATG
Summary
The main objectives of this study were to determine the chromosomal location of the PLC genes in wheat and survey the prevalence of a frame shift mutation identified in one Ta-PG-PLC gene, while sequencing, among twenty-one wheat cultivars and in progenitor species
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
