Gene introgression from common wheat into Aegilops L.

Abstract

Group of experiments were carried out to verify possibility of gene introgression from common wheat into Aegilops. The artificial indoor crossbreed was conducted using 7 genotypes from 4 wheat relative species as female, and common wheat as male. The experiment result shows that different species has variable cross ability. Among the 4 Aegilops species, the highest cross rate is from the combination of Aegilops tauschii × Triticum aestivum (46.49% for genotype Ae42, 22.58% for Y92), the second is from Aegilops ovata × T. aestivum (14.76% for Y100, 12.11% for Ae23), the third is from Aegilops cylindrica × T. aestivum (2.23% for Ae7, 8.50% for Y145), and the lowest is from Aegilops speltoides × T. aestivum (0.19%). Hybrid embryos from different combinations have different ability of callus initiation and germination. The hybrid embryos from A. ovata/T. aestivum and Ae. tauschii/T. aestivum have a higher level of callus initiation and germination. Ae. cylindrica/T. aestivum has a middle level, while the Ae. speltoides has a lower level. The interspecific hybrids between Aegilops and common wheat have so low fertility. In back-crosses, the seed-set rate of hybrids of Ae. ovata/T. aestivum is 3.71% and 4.36% respectively back-crossed with male and female parents, while for hybrids of Ae. cylindrica/T. aestivum, they were 0 and 0.33% respectively, and for Ae. tauschii/T. aestivum, 0.33% and 0 respectively. On selfing of the hybrids, the seed-set rate is 0 (no seed set from 9750 florets) for the combination of Ae. cylindrica/T. aestivum, 0.044% (3 selfed seeds out of 6870 florets) for Ae. ovata/T. aestivum and 0 (no seed set from 7253 florets) for Ae. tauschii/T. aestivum. The research suggests that the probability of gene introgression from T. aestivum into Aegilops species is very low in nature.