Gene frequencies of human platelet antigen‐1 to ‐5 and ‐15 in the Iranian population

Abstract

BackgroundAs the frequency of human platelet antigens varies between different ethnic groups and the data about the HPA gene frequency in Iran have been absent yet, the purpose of this study was to determine HPA‐1 to HPA‐5 and HPA‐15 allele frequency among Iranians.Materials and methodsHPA‐1, HPA‐2, HPA‐3, HPA‐4, HPA‐5 and HPA‐15 typing was performed by the polymerase chain reaction–sequence‐specific primer techniques (PCR‐SSP) for 210 subjects [Including 100 blood donors, 55 hematopoietic stem cell (HSC) recipient/donor pairs].ResultsThe frequencies of HPA phenotypes were determined as follows: HPA‐1a/1a: 98%, HPA‐1a/1b:2%, HPA‐2a/2a: 8/.5%, HPA‐2a/2b: 91·5%, HPA‐3a/3a: 21%, HPA‐3a/3b: 67·2%, HPA‐3b/3b: 22·8%, HPA‐4a/4a: 100%, HPA‐5a/5a: 99·5%, HPA‐5a/5b: 0·5%, HPA‐15a/15a: 14·2%, HPA‐15a/15b: 67·2% and HPA‐15b/15b: 18·6%.ConclusionHPA‐4b was absent and there was not any homozygosity for HPA‐1b/1b, HPA‐2b/2b and HPA‐5b/5b besides no HPA‐1b/b, HPA‐2b/b and HPA‐5b/b homozygosity was found. So it seems that HPA‐1a, HPA‐2a, HPA‐4a and HPA‐5a might not be involved in the alloimmunization and transfusion reactions on the Iranian population but HPA‐15a and HPA3a. Furthermore, extensive studies covering diverse ethnic groups, in the field of platelet immunobiology, will help to find the local pattern more precisely.