Gene expression tryptophan aspartate coat protein in determining latent tuberculosis infection using immunocytochemistry and real time polimerase chain reaction.

Rebekah J Setiabudi,Wayan Tunas Artama,Muhammad Amin,Ni Made Mertaniasih

doi:10.4081/idr.2020.8733

Rebekah J Setiabudi, Wayan Tunas Artama + Show 2 more

Open Access

https://doi.org/10.4081/idr.2020.8733

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Abstract

BackgroundTuberculosis (TB) remains a major cause of morbidity and mortality worldwide. Problem of Latent Tuberculosis Infection (LTBI) is increasing in number especially in countries with high TB incidence rate, such as Indonesia. Although not every LTBI will become active TB, if untreated and not handled appropriately it can still be a source of transmission and may increase the rate of resistance to the first-line TB drugs. Mycobacterium tuberculosis as a cause of tuberculosis disease is an intracellular pathogens that survives within the phagosome of host macrophages. Several host factors are involved in this process, including the Tryptophan Aspartate-containing Coat Protein (TACO). TACO is a protein recruited and retained by viable Mycobacterium tuberculosis on the surface of the phagosome membrane to maintain its survival in phagosome, because the presence of TACO plays an important role in inhibiting the fusion of phagosomes and lysosomes.Objectivethe aim of this studyis to assess the difference of gene expression TACO protein in Latent Tuberculosis Infection (LTBI) and healthy people.MethodA preliminary studyof mRNA examination of TACO protein using Immunocytochemistry (ICC) and Real Time-Polimerase Chain Reaction (RT-PCR) method by a PCR Light Cycler 2.0 machine (Roche) in LTBI and healthy groups.Results18 samples of peripheral blood monocyte cells (PBMCs) were collected and divided into 2 groups. We found that there was a significantly difference between the 2 groups of samples.ConclusionFurther research is required to consider that the measurement of TACO expression using RT-PCRcan used as one of the other method to determine LTBI.

Highlights

Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide
18 samples of peripheral blood gens present on the surface of and multiply in macrophage cells
We found that occurs through various receptors present in macrophage cells and how they can cause there was a significantly difference between the macrophages, including mannose recep- Mycobacterium tuberculosis to survive in the 2 groups of samples

Summary

Introduction

Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide. Currently reported, one-third of theKey words: tryptophan aspartate containing coat protein, immunocytochemistry, real timepolimerase chain reaction, Mycobacterium tuberculosis, latent tuberculosis infection.1,2Department of Medical Microbiology, TB Laboratory Institute of Tropical Disease; 3Department of Pulmonology, Faculty of Medicine, Universitas Airlangga; 4Faculty of Veterinary, Gajah Mada University, Indonesia entire world’s population has been infected by Mycobacterium tuberculosis, the cause of tuberculosis. Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide. 1,2Department of Medical Microbiology, TB Laboratory Institute of Tropical Disease; 3Department of Pulmonology, Faculty of Medicine, Universitas Airlangga; 4Faculty of Veterinary, Gajah Mada University, Indonesia entire world’s population has been infected by Mycobacterium tuberculosis, the cause of tuberculosis. By 2015 it is estimated that there are 10.4 million people worldwide suffering from clinical TB, 5.9 million (56%) are male, 3.5 million (34%) females and 1.2 million (11%) children. There are Contributions: All the authors contributed . Conflict of interest: The authors declare no potential conflict of interest

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