Gene expression responses to restricted feeding and extracted soybean meal in Atlantic salmon (Salmo salar L.)

Abstract

We assessed effects of feed restriction and inclusion of 200 g kg−1 extracted soybean meal in the diet on gene expression in Atlantic salmon using a cDNA microarray (SFA2.0) and real-time qPCR. The trial lasted for 54 days. Restricted feeding and soybean inclusion reduced the thermal growth coefficient by respectively 51% and 22% compared with fish fed with the fishmeal-based control diet to satiation. Soybean decreased distal intestinal expression of lysosomal (cathepsins C, D, L, Y and Z) and extracellular proteases while genes involved in responses to cellular stress were up-regulated. Expression changes of immune genes suggested both pro- and anti-inflammatory regulation. The hepatic responses to soybean and restricted feeding were highly similar, which could be because of negative effects of soybean meal on digestion and nutrient absorption. We observed up-regulation of ribosomal proteins and down-regulation of genes involved in lipid and steroid metabolism. Of note, growth reduction in both study groups was associated with coordinated down-regulation of genes involved in oxidative and cellular stress responses, metabolism of xenobiotics and protein degradation. High expression of stress-related genes in salmon fed with the control diet suggests that maximum growth rates can be associated with health problems.