Gene expression proles of miR-1908 over-expressed human multipotent adipose-derived stem cells by cDNA microarrays

Abstract

Objective To explore the underlying molecular basis of hsa-miR-1908 in obesity, and to exami-ne human multipotent adipose-derived stem (hMADS) cell genes differentially expressed in hsa-miR-1908 over-expressed hMADS cell by using cDNA microarrays. Methods A lentiviral vector containing miR-1908 with high overexpression in the infected hMADS cells was constructed.Gene expression proles of hsa-miR-1908 over-expressed human multipotent adipose-derived stem cells were obtained by cDNA microarrays.Three of the differentially regulated genes identified with cDNA microarray analysis were randomly selected for validation analysis by Real-time quantitative PCR. Results cDNA microarrays containing 34 127 genes were used to investigate gene expression of hMADS cells.The analysis of gene expression proles indicated that 69 genes were up-regulated and 54 genes were down-regulated in hsa-miR-1908 over-expressed hMADS cells.Based on their molecular functions, these genes were classified into 15 different groups, including macromolecular complex subunit organization (18/123, 14.63%), RNA binding (16/123, 13.01%), phosphatase activity (9/123, 7.32%), protein domain specific binding (10/123, 8.13%), phosphoprotein phosphatase activity (8/123, 6.50%), cellular component disassembly (5/123, 4.07%), kidney development (6/123, 4.88%), transcription from RNA polymerase Ⅱ promoter (9/123, 7.32%), urogenital system development (6/123, 4.88%), transcription DNA-dependent (9/123, 7.32%), RNA biosynthetic process (9/123, 7.32%), apoptosis (14/132, 11.38%), programmed cell death (14/123, 11.38%), type Ⅰ interferon biosynthetic process (2/123, 1.63%), and type Ⅰ interferon production (2 /123, 1.63%). Conclusions The present study provides a new view on the study of hsa-miR-1908, unraveling some of the molecular events taking place in hMADS cells, adding candidate genes for future investigation. Key words: MicroRNA-1908; Multipotent adipose-derived stem cells; cDNA microarrays; Obesity