Abstract
Chromosomal translocations are genetic aberrations associated with specific non-Hodgkin lymphoma (NHL) subtypes. This study investigated the differential gene expression profile of Egyptian NHL cases based on a microarray approach. The study included tissue samples from 40 NHL patients and 20 normal lymph nodes used as controls. Total RNA was extracted and used for cDNA microarray assays. The quantitative real time polymerase chain reaction was used to identify the aberrantly expressed genes in cancer. Significant associations of 8 up-regulated and 4 down-regulated genes with NHL were observed. Aberrant expression of a new group of genes not reported previously was apparent, including down-regulated NAG14 protein, 3 beta hydroxy-delta 5-c27 steroid oxi-reductase, oxi-glutarate dehydrogenase (lipo-amide), immunoglobulin lambda like polypeptide 3, protein kinase x linked, Hmt1, and caveolin 2 Tetra protein. The up-regulated genes were Rb binding protein 5, DKFZP586J1624 protein, protein kinase inhibitor gamma, zinc finger protein 3, choline ethanolamine phospho-transferase CEPT1, protein phosphatase, and histone deacetylase-3. This study revealed that new differentially expressed genes that may be markers for NHL patients and individuals who are at high risk for cancer development.
Highlights
Malignant lymphomas are genetically characterized by distinctive chromosomal translocations as the t (14;18) in follicular lymphoma
The current study from 40 cancerous tissues from non–Hodgkin lymphoma (NHL) showed common genetic variation in cell cycle, apoptosis, and lymphocyte development regulatory genes that may play a role in lymphomagenesis
A study shows that the target genes differentially expressed in Diffuse large B-cell lymphoma (DLB-CL) include BCL-6, B lymphocyte-induced maturation protein 1 (BLIMP1), and X-box-binding protein 1 (XBP1) (Staudt and Dave, 2005).The current study showed most aberrations genes are: BCL-6, and BLIMP1, and XBP1, MYC and CCND1
Summary
Malignant lymphomas are genetically characterized by distinctive chromosomal translocations as the t (14;18) in follicular lymphoma. Non–Hodgkin lymphomas (NHL) diseases are involving malignant transformation of lymphoid cells. Specific Chromosomal translocations often associated with NHL subtypes (Dyer, 2003; Kuppers, 2005; Ohno, 2006; Bende et al, 2007). NHL-associated translocations result in transcriptional deregulation of proto-oncogene or oncogene (Dyer, 2003; Kuppers, 2005; Ohno, 2006; Bende et al, 2007). Chromosomal translocations are genetic aberrations associated with specific non–Hodgkin lymphoma (NHL) subtypes. This study investigated the differential gene expression profile of Egyptian NHL cases based on a microarray approach. Conclusions: This study revealed that new differentially expressed genes that may be markers for NHL patients and individuals who are at high risk for cancer development
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