Abstract
cDNA microarrays were used to compare the gene expression pattern of a nonmetastatic colorectal cancer cell line (SW480) with its metastatic derivative (SW620). Co-hybridization of euores- cently labeled cDNA generated from SW480 (Cy3) and SW620 (Cy5) total RNA to microarrays containing 4000 human cDNA clones revealed differential expression of 129 genes involved in the regulation of tran- scription, cell-cycle control and division, cell signaling, cell adhesion, and cell metabolism. The results of this microarray analysis corresponded to previously reported gene expression proeling experiments with SW480 and SW620 using SAGE. Predictably, the metastatic cell line SW620 exhibited underexpression of genes involved in cell adhesion and overexpression of genes involved in transcription and translation compared with its nonmetastatic counterpart SW480. Finally, by applying a novel bioinformatics approach to the gene expression data, a number of genes underexpressed in SW620 compared with SW480 were demonstrated to map to chromosomal band 17q21-23, a region that may be associated with loss of heterozygosity during the metastatic progression of colorectal cancer.
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