Gene Expression Profiling of Corpus luteum Reveals Important Insights about Early Pregnancy in Domestic Sheep.

Kisun Pokharel,Melak Weldenegodguad,Jaana Peippo,Meng-Hua Li,Mervi Honkatukia,Juha Kantanen

doi:10.3390/genes11040415

Kisun Pokharel, Melak Weldenegodguad + Show 4 more

Open Access

https://doi.org/10.3390/genes11040415

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Abstract

The majority of pregnancy loss in ruminants occurs during the preimplantation stage, which is thus the most critical period determining reproductive success. Here, we performed a comparative transcriptome study by sequencing total mRNA from corpus luteum (CL) collected during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287 genes were expressed in our data. Highly expressed autosomal genes in the CL were associated with biological processes such as progesterone formation (STAR, CYP11A1, and HSD3B1) and embryo implantation (e.g., TIMP1, TIMP2 and TCTP). Among the list of differentially expressed genes, sialic acid-binding immunoglobulin (Ig)-like lectins (SIGLEC3, SIGLEC14, SIGLEC8), ribosomal proteins (RPL17, RPL34, RPS3A, MRPS33) and chemokines (CCL5, CCL24, CXCL13, CXCL9) were upregulated in Finnsheep, while four multidrug resistance-associated proteins (MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized non-coding RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the flushing diet effect. The significantly upregulated TXNL1 gene indicated potential for embryonic diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes that are active in the CL during early pregnancy (including TXNL1, SIGLEC14, SIGLEC8, MRP4, and CA5A).

Highlights

Litter size, a key determinant for the profitability of sheep production systems, is highly dependent on ovulation rate and embryo development in the uterus
Our phenotypic records indicate lower embryo survival rate in Finnsheep compared to Texel
We identified several key transcripts, including coding genes and noncoding genes, that are essential during early pregnancy

Summary

Introduction

A key determinant for the profitability of sheep production systems, is highly dependent on ovulation rate and embryo development in the uterus. The native Finnsheep, one of the most highly prolific breeds, has been exported to more than 40 countries to improve local breeds [9]. Mutation in gene GDF9 has been identified to be strongly associated with litter size in Finnsheep and breeds such as the Norwegian White Sheep, Cambridge and Belclare breeds, which were developed using Finnsheep [10,11,12,13]. The preimplantation stage of pregnancy is the most critical period in determining the litter size because of the high embryo mortality during this period. The CL is an endocrine structure whose main function is to synthesize and secrete the hormone progesterone

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