Abstract
BackgroundThe studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells.ResultsWe isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non-side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip® oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (AKR1C1/C2), Transmembrane 4 L six family member 1 nuclear receptor (TM4SF1), and Nuclear receptor subfamily 0, group B, member 1 (NR0B1) were significantly up-regulated in SP compared to non-SP cells.ConclusionThis is the first report indicating the differences of gene expression pattern between SP and non-SP cells in A549 cells. We suggest that the up-regulations of the genes AKR1C1/C2, TM4SF1 and NR0B1 in SP of human adenocarcinoma A549 cells could be a target of poor prognosis in anti-cancer therapy.
Highlights
The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications
Since we focused on distinct gene regulations, the student's t'-test was not employed to prevent loss of up-regulated genes in all of three chip data, though it had large chip variations
Based on the cancer stem cell hypothesis, we assumed that the up-regulation of certain genes that are related to poor prognosis in side population (SP) of cancer cells could be a target for therapeutic index
Summary
The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. Tissue-specific stem cells are defined by their ability to self-renew and to produce the well differentiated and functional cells within an organ. Molecular Cancer 2007, 6:75 http://www.molecular-cancer.com/content/6/1/75 generally short-lived; in skin and blood for example, they are produced from a small pool of long-lived stem cells that last throughout the life [3,4,5,6]. Stem cells are necessary for tissue development, replacement, and repair [7]. The longevity of stem cells make them susceptible to accumulating genetic damage and thereby representing the growth root for cancer recurrence following treatment [8]. It was reported that some of the tumor stem cells can survive chemotherapy and support re-growth of the tumor mass [9]
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