Gene Expression Profiling in Peripheral Blood in Ruptured Intracranial Aneurysm (S23.006)

Abstract

Objective: To characterize a systemic response to subarachnoid hemorrhage from a ruptured intracranial aneurysm (IA) through analysis of global gene expression profiles in peripheral blood. Background Subarachnoid hemorrhage (SAH) from a ruptured IA remains a serious medical, social and economic problem. Molecular mechanism underlying the systemic response to this life-threaten condition are not fully understood. Most studies performed in human patients were focused on pre-defined pathways. Design/Methods: Peripheral blood samples were collected from n=43 adult patients with ruptured IAs and n=18 adult control subjects without IAs. Among IAs patients blood was collected on days 0.-1. in n=21 cases, on days 2.-3. in n=6 cases, and on day 4. or later in n=16 cases. Gene expression profiling was performed using Illumina HumanHT-12 v4 microarrays. Quantitative reverse-transcription polymerase chain reaction was used as a confirmatory test for some of the differentially expressed genes. Functional analysis for determination of over-represented ontological groups among differentially expressed gene was performed. Institutional IRB approved the study. Results: There were 189 differentially expressed genes in ruptured IAs subjects comparing with controls (p Conclusions: Results of the current study indicate that the rupture of IAs strongly influences the transcription profiles of peripheral blood cells. A specific pattern of these expression changes was found suggesting a depression of lymphocytes response and an enhancement of monocytes and neutrophiles activity. This finding is probably related to a immunodepression observed in SAH patients. Supported by: The Polpharma Scientific Foundation (3/30/VIII/2009) and DeMeTer 3.1. Disclosure: Dr. Pera has nothing to disclose. Dr. Korostynski has nothing to disclose. Dr. Piechota has nothing to disclose. Dr. Golda has nothing to disclose. Dr. Krupa has nothing to disclose. Dr. Moskala has nothing to disclose. Dr. Przewlocki has nothing to disclose. Dr. Szczudlik has nothing to disclose. Dr. Slowik has nothing to disclose.