Gene expression profiling during spermatogenesis in early maturing male Atlantic salmon parr testes

Abstract

The initiation of sexual maturation and spermatogenesis are complex processes that require the highly coordinated regulation of a number of key genes. The endocrine system plays crucial roles in these processes, but the precise mechanisms involved in sexual maturation of fish are poorly understood. We investigated the expression of genes encoding proteins involved in sex steroid biosynthesis (Ff1b (FTZ-F1 homolog), steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), 3β-hydroxysteroid dehydrogenase/Δ 5–Δ 4–isomerase (3β-HSD), cytochrome P450 17α-hydroxylase/17,20-lyase (P450c17), cytochrome P450 11β-hydroxylase (P45011β) and 11β-hydroxysteroid dehydrogenase (11β-HSD)) and the anti-Müllerian hormone (AMH) homolog during early sexual maturation of one-summer-old male Atlantic salmon parr by RT-PCR. Genes encoding Ff1b, StAR, 3β-HSD, P450c17 and 11β-HSD were upregulated during spermatogonial proliferation. During the course of spermatogenesis expression profiles of Ff1b, StAR, 3β-HSD, P450scc, P450c17, P45011β, and 11β-HSD were similar; transcript levels being low during early stages, then strongly increasing during spermiogenesis. These results indicate that coordinated de novo transcription of genes encoding StAR as well as 3β-HSD, P450c17 and 11β-HSD might be required for sex steroids production during the initiation of spermatogenesis in salmon. In contrast, transcription levels of AMH were comparatively high in immature testes, decreased when spermatogenesis was initiated, and were lowest during spermiogenesis, suggesting that AMH suppression plays a crucial role in the process of spermatogenesis in salmonids. Correlation analyses show that FSH and LH might be differentially involved in the regulation of several of these genes studied.