Abstract
Bisphenol A (BPA) is an environmental endocrine disruptor which has been detected in human bodies. Many studies have implied that BPA exposure is harmful to human health. Previous studies mainly focused on BPA effects on estrogen receptor (ER)-positive cells. Genome-wide impacts of BPA on gene expression in ER-negative cells is unclear. In this study, we performed RNA-seq to characterize BPA-induced cellular and molecular impacts on ER-negative HEK293 cells. The microscopic observation showed that low-dose BPA exposure did not affect cell viability and morphology. Gene expression profiling analysis identified a list of differentially expressed genes in response to BPA exposure in HEK293 cells. These genes were involved in variable important biological processes including ion transport, cysteine metabolic process, apoptosis, DNA damage repair, etc. Notably, BPA up-regulated the expression of ERCC5 encoding a DNA endonuclease for nucleotide-excision repair. Further electrochemical experiment showed that BPA induced significant DNA damage in ER-positive MCF-7 cells but not in ER-negative HEK293 cells. Collectively, our study revealed that ER-negative HEK293 cells employed mechanisms in response to BPA exposure different from ER-positive cells.
Highlights
Bisphenol A (BPA) is an important industrial chemical mainly used as an intermediate in the manufacture of polycarbonate plastics and epoxy resin
To assess cellular and molecular effects induced by BPA exposure of environmental relevant concentration, we investigated the toxicity of low-dose (1026 M) BPA exposure on human embryonic kidney 293 cells (HEK293) cells
After 48 h treatment, we examined the effects of BPA exposure on cell morphology and transcriptome profile
Summary
Bisphenol A (BPA) is an important industrial chemical mainly used as an intermediate in the manufacture of polycarbonate plastics and epoxy resin. The genome-wide impacts of BPA exposure on gene expression especially in ER-negative cells is yet to be uncovered. To characterize the cellular and molecular effects of BPA on ER-negative cells, we performed RNA-seq to examine perturbation on gene expression exerted by low-dose BPA in HEK293 cells.
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