Gene Expression Profiles Deciphering the Pathways of Coronatine Alleviating Water Stress in Rice (Oryza sativa L.) Cultivar Nipponbare (Japonica).

Wei Gao,Lin Ai,Chunxin Yu,Yuyi Zhou,Liusheng Duan

doi:10.3390/ijms20102543

Wei Gao, Lin Ai + Show 3 more

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https://doi.org/10.3390/ijms20102543

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Abstract

Coronatine (COR) is a structural and functional analog of methyl jasmonic acid (MeJA), which can alleviate stress on plant. We studied the effects of COR on the drought stress of rice (Oryza sativa L.). Pre-treatment with COR significantly increased the biomass, relative water and proline content, and DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, decreased the electrolyte leakage and MDA (Malondialdehyde) content in order to maintain the stability of cell membrane. Meanwhile, we determined how COR alleviates water stress by Nipponbare gene expression profiles and cDNA microarray analyses. Seedlings were treated with 0.1 μmol L−1 COR at the three leafed stage for 12 h, followed with 17.5% polyethylene glycol (PEG). Whole genome transcript analysis was determined by employing the Rice Gene Chip (Affymetrix), a total of 870 probe sets were identified to be up or downregulated due to COR treatment under drought stress. Meanwhile, the real-time quantitative PCR (RT-qPCR) method was used to verify some genes; it indicated that there was a good agreement between the microarray data and RT-qPCR results. Our data showed that the differentially expressed genes were involved in stress response, signal transduction, metabolism and tissue structure development. Some important genes response to stress were induced by COR, which may enhance the expression of functional genes implicated in many kinds of metabolism, and play a role in defense response of rice seedling to drought stress. This study will aid in the analysis of the expressed gene induced by COR.

Highlights

Rice (Oryza sativa L.) is widely cultivated as a staple crop throughout the world, and it is a model monocot plant for molecular and genetic studies, while water deficiency is one of the most important limitations to its growth and grain yield
As may be seen from the photo of Nipponbare taken at 48 h after 17.5% polyethylene glycol (PEG) treatment (Figure 1), the changes of rice with different treatments were remarkable
At 7 d after PEG treatment, the dry weight of seedlings was significantly reduced by 37.6% for Nipponbare (Table 1), the fresh mass of rice treated with COR increased 83.7% under drought stress, while COR had no effect on seedling weight before the initiation of drought treatment

Summary

Introduction

Rice (Oryza sativa L.) is widely cultivated as a staple crop throughout the world, and it is a model monocot plant for molecular and genetic studies, while water deficiency is one of the most important limitations to its growth and grain yield. Shortage of water limits plant growth and crop productivity more than any other single environmental factor [1]. Drought and water deficit can decrease photosynthetic capacity, result in dehydration of plant cells, oxidative damage to chloroplasts, imbalance of photosynthesis and respiration, limit metabolic reactions, and reduce dry matter accumulation and partitioning [2,3]. Drought stress may trigger the formation of superoxide radical and hydrogen peroxide (H2O2), which were paralleled by malondialdehyde (MDA) accumulation in rice [6]. The 2,2-dipenyl-1- picrylhydrazyl (DPPH)-ridical scavenging activity is closely correlated with stress tolerance [8], and has been accepted as a measure of radical-scavenging activity [9]

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