Abstract
BackgroundBaculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines.ResultsThe analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and “simplified” experimental procedure.ConclusionsThe results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.
Highlights
Dendritic cells (DCs) are professional antigen-presenting cells (APC) able to initiate immune responses [1,2]
The expression of all the four markers was upregulated in monocyte-derived dendritic cells (MDDCs) as well as in CD14 – expressing peripheral blood mononuclear cells (PBMCs) by treatment with HIV-Virus-like particles (VLPs), compared to PBS (Fig.1)
The results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed in CD14-expressing freshly derived PBMCs
Summary
Dendritic cells (DCs) are professional antigen-presenting cells (APC) able to initiate immune responses [1,2]. Antigen-loaded DCs acquire a mature phenotype, associated with reduced endocytic and phagocytic capacities [3,4,5,6], and migrate toward the lymphoid organs to activate naïve T cells, through upregulated costimulatory molecules such as (page number not for citation purposes). There are two main DC types in human peripheral blood, known as myeloid DCs (mDCs), the major subset representing around 80% of blood DCs [11], and plasmacytoid DCs (pDCs). Considering that DCs represent only 1-3% of peripheral blood mononuclear cells (PBMCs), immature DCs can be obtained in vitro from peripheral blood monocytes, generating monocytederived DCs (MDDCs) [12]. Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1and Th2-specific cytokines
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